A simple chemiluminescence method for the sensitive quantitation of SEAP
Features
  • A simple chemiluminescence assay for measuring SEAP from transfected cells
  • Detect SEAP activity in culture medium in the mU/well range
  • Contains enough reagents to run three 96-well plates
  • Rapid assay; get results within 1 hour
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Product Type

Assay & Reagent Kits
Assay Kits
Enzyme Activity

Research Area

Application

Chemiluminescence
Enzyme Activity

Secreted Alkaline Phosphatase Reporter Gene Assay Kit (Luminescence)

Item No. 600260

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • SEAP Reporter Gene
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    300 tests

    $329

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    Product Description

    Secreted alkaline phosphatase (SEAP) is commonly used as a reporter of gene expression. Compared to other conventional intracellular reporters such as chloramphenicol acetyltransferase (CAT) and firefly luciferase, SEAP has the advantage of being secreted from transfected cells into the culture medium. Cayman’s Secreted Alkaline Phosphatase Reporter Gene Assay Kit (Luminescence) provides a simple chemiluminescence method for the sensitive quantitation of SEAP in conditioned cell culture medium from transfected cells. The assay can detect SEAP activity in the milliunits/well (mU/ml) range. The kit includes enough reagents to run three 96-well plates. The assay is easy to perform and can be completed within one hour.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.