EP₄ Receptor (rat) Reporter Assay Kit

The EP₄ receptor is one of four GPCRs that mediate the actions of prostaglandin E₂ (PGE₂). The diverse effects of PGE₂ acting via EP₄ receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman's EP₄ Receptor (rat) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for rat EP₄ receptor and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (EP₄ Receptor Reverse Transfection Strip Plate). Cells grown on the transfection complex will express EP₄ at the cell surface. Binding of agonists to EP₄ initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture media. Aliquots of media are removed at time intervals, beginning at approximately six hours, and SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is simple to use and can be easily adapted to high throughput screening for therapeutic compounds regulating the activation of EP₄. PGE₂ is included in the kit for use as a positive control. The kit provides sufficient reagents to measure SEAP activity at three time points using the black plates included.

WARNING This product is not for human or veterinary use.