A cell-based screening assay based on novel transfection technology
Features
  • Reverse transfection reporter assay to screen for EP4 receptor agonists
  • Assay 18 samples in duplicate
  • ELISA-based cAMP measurement
  • Plate-based colorimetric measurement (405-420 nm)
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EP4 Receptor (rat) Activation Assay Kit (cAMP)

Item No. 600410

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Limit of Detection
3.1 pmol/ml
Sensitivity
50% B/B0: 20.4 pmol/ml
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea

    $576

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    Product Description

    The EP4 receptor is one of four GPCRs that mediate the actions of prostaglandin E2 (PGE2). The diverse effects of PGE2 acting via EP4 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman's EP4 Receptor (rat) Activation Assay Kit (cAMP) consists of a 96-well plate coated with a transfection complex containing a DNA construct for rat EP4 Receptor (EP4 Reverse Transfection Strip Plate). Cells grown on the transfection complex will express EP4 at the cell surface. Binding of agonists to EP4 stimulates cAMP generation and increases intracellular cAMP levels, which can be measured by a competitive EIA using the Mouse Anti-Rabbit IgG Coated Plate and the reagents included in the kit. An EP4 agonist, PGE2, is included in the kit as a positive control.

    WARNING This product is not for human or veterinary use.