A colorimetric-based method for detecting L-lactate in culture medium
Features
  • Detect L-lactate, the end product of glycolysis
  • Can be adapted to high-throughput screening
  • Measure L-lactate in cell culture supernatant down to 156 µM
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Glycolysis Cell-Based Assay Kit

Item No. 600450

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea

    $380

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    Product Description

    Cayman’s Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting L-lactate, the end product of glycolysis, produced and secreted by cultured cells. In this assay, lactate dehydrogenase (LDH) catalyzes the reaction between NAD+ and lactate, yielding pyruvate and NADH. The NADH directly reduces a tetrazolium salt (INT) to a colored formazan which absorbs between 490 and 520 nm. The quantity of formazan produced is proportional to the quantity of lactate in the culture medium and is thus an indirect measurement of glycolysis. This assay can be adapted to high-throughput screening to examine glycolytic regulators in a cell-based system. In addition, the non-invasive sampling leaves the cells intact and thus allows multiplex testing for additional markers.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Kumari, S., Khan, S., Gupta, S.C., et al. MUC13 contributes to rewiring of glucose metabolism in pancreatic cancer. Oncogenesis 7:19, (2018).

    Gomez de Cedrón, M., Acin Pérez, R., Sánchez-Martínez, R., et al. MicroRNA-661 modulates redox and metabolic homeostasis in colon cancer. Mol. Oncol. 11(12), 1768-1787 (2017).

    Sakamoto, S., Miyara, M., Sanoh, s., et al. Mild MPP+ exposure-induced glucose starvation enhances autophagosome synthesis and impairs its degradation. Sci. Rep. 7, 46668 (2017).