A colorimetric method for measuring intracellular NAD/NADH in culture cells
Features
  • Measure total cellular NAD
  • Assay 38 samples in duplicate
  • Assay Range: 15.6 nM to 1 µM
  • Plate-based colorimetric measurement (450 nm)
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NAD/NADH Cell-Based Assay Kit

Item No. 600480

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Nicotinamide adenine dinucleotide/Nicotinamide adenine dinucleotide, reduced
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea

    $413

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    Product Description

    Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form, NAD+, as well as a reduced form, NADH. NAD, the main free form in cells, functions in modulating cellular redox status and by controlling signaling and transcriptional events, making it an important cofactor when investigating normal cellular function. Cayman’s NAD/NADH Cell-Based Assay Kit provides a colorimetric method for measuring intracellular NAD+ and NADH in cultured cells. In this assay, NAD+ found in cell samples is reduced to NADH by alcohol dehydrogenase during the oxidation of ethanol to acetaldehyde. The newly formed and the existing NADH found in the samples is then oxidized resulting in the reduction of a tetrazolium salt substrate (WST-1) to a highly-colored formazan which absorbs at 450 nm. The amount of formazan produced is proportional to the amount of total NAD in the cell lysate and can be used as an indicator of the total cellular NAD concentration.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Ting, K.K.Y., Ibrahim, H.M., Gulati, N., et al. Intracellular accumulation of free cholesterol in macrophages triggers a PARP1 response to DNA damage and PARP1 impairs lipopolysaccharide-induced inflammatory response. PLoS One 20(3), e0318267 (2025).

    Heden, T.D., Chow, L.S., Hughey, C.C., et al. Regulation and role of glycophagy in skeletal muscle energy metabolism. Autophagy 18(5), 1078-1089 (2022).

    Huang, P., Lee, M.W.J., Sadrerafi, K., et al. MC-PPEA as a new and more potent inhibitor of CLP-induced sepsis and pulmonary inflammation than FK866. Drug Des. Devel. Ther. 11, 629-641 (2017).

    Funk, R.S., Singh, R., Pramann, L., et al. Nicotinamide phosphoribosyltransferase attenuates methotrexate response in juvenile idiopathic arthritis and in vitro. Clin. Transl. Sci. 9(3), 149-157 (2016).