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Neutrophil Elastase Activity Assay Kit

Item № 600610
product image
     2 x 96 wells $350.00 0.00

Pricing updated 2019-07-21. Prices are subject to change without notice.

  • Neutrophils isolated from whole blood (protocol included)
  • Measure neutrophil elastase released by neutrophils after various experimental conditions
  • Employs the specific elastase substrate (Z-Ala-Ala-Ala-Ala)2Rh110
  • Includes reagents to isolate neutrophils from whole blood and PMA to stimulate elastase release
  • Assay performed in provided black 96-well plates
  • Neutrophil (mouse) Isolation Kit (Item No. 601070) available to isolate mouse neutrophils from peritoneal lavage or bone marrow

Neutrophil elastase is stored within cytoplasmic azurophilic granules in the neutrophil and released upon stimulation by pathogens where it acts either as free protein or is associated with networks of extracellular traps (NET). Together with other proteases released from activated neutrophils, neutrophil elastase plays a critical role in degrading invading pathogens and thus provides the earliest line of defense in the immune system. It is thought to play a critical role in tumor invasion and metastasis as well as other inflammatory conditions. Cayman’s Neutrophil Elastase Activity Assay kit is designed to be used to study compounds regulating elastase release in neutrophils. The kit employs a specific non-fluorescent elastase substrate, (Z-Ala-Ala-Ala-Ala)2Rh110, which is selectively cleaved by elastase to yield the highly fluorescent compound R110, which can be analyzed with an excitation wavelength of 485 nm and emission wavelength of 525 nm. Reagents needed to isolate neutrophils from whole blood are included in the kit, as is PMA, which is known to stimulate elastase release from neutrophils.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

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