A convenient all-in-one tool to measure neutrophil elastase activity
Features
  • Neutrophils isolated from whole blood (protocol included)
  • Measure neutrophil elastase released by neutrophils after various experimental conditions
  • Employs the specific elastase substrate (Z-Ala-Ala-Ala-Ala)2Rh110
  • Includes reagents to isolate neutrophils from whole blood and PMA to stimulate elastase release
  • Assay performed in provided black 96-well plates
  • Neutrophil (mouse) Isolation Kit (Item No. 601070) available to isolate mouse neutrophils from peritoneal lavage or bone marrow
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Neutrophil Elastase Activity Assay Kit

Item No. 600610

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    2 x 96 wells

    $494

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    Product Description

    Cayman’s Neutrophil Elastase Activity Assay kit employs a specific non-fluorescent elastase substrate, (Z-Ala-Ala-Ala-Ala)2Rh110, which is selectively cleaved by elastase to yield the highly fluorescent compound R110. Fluorescence can be analyzed with an excitation wavelength of 485 nm and emission wavelength of 525 nm. Reagents needed to isolate neutrophils from whole blood are included in the kit, as is PMA, which is known to stimulate elastase release from neutrophils. The assay can be used to study compounds regulating elastase release in neutrophils. If a cell line known to express neutrophil elastase is used, a proper control using a specific neutrophil elastase inhibitor, such as ONO-6818 which is not provided, should be included to ensure specificity of the enzyme.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.