A complete assay for the isolation of neutrophils and measurement of MPO activity
Features
  • Assay the release of enzymatically active MPO from activated phagocytes
  • Utilizes TMB as a chromogenic substrate for MPO
  • Includes a specific inhibitor of MPO function to verify specificity
  • Includes reagents needed to isolate neutrophils from human whole blood
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Neutrophil Myeloperoxidase Activity Assay Kit

Item No. 600620

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Neutrophil MPO
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    2 x 96 wells

    $398

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    Product Description

    Cayman’s Neutrophil Myeloperoxidase Activity Assay kit utilizes 3,3’,5,5’-tetramethyl-benzidine (TMB) as a chromogenic substrate, which upon reacting with MPO, yields a blue color detectable by its absorbance at 650 nm. The color intensity is proportional to the amount of MPO in the sample. Reagents needed to isolate neutrophils from whole blood are included in the kit, as is PMA, which is known to stimulate MPO release from neutrophils. A specific inhibitor for MPO, 4-aminobenzhydrazide (ABH), is also included in the kit for verifying the specificity of the assay.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Basu, J., Olsson, A., Ferchen, K., et al. ThPOK is a critical multifaceted regulator of myeloid lineage development. Nat. Immunol. 24(8), 1295-1307 (2024).

    Nishikido, T., Oyama, J., Shiraki, A., et al. Deletion of apoptosis inhibitor of macrophage (AIM)/CD5L attenuates the inflammatory response and infarct size in acute myocardial infarction. J. Am. Heart Assoc. 5(4), e002863 (2016).