A non-radioactive method for measuring protein synthesis
Features
  • Non-radioactive, simple procedure
  • Measure nascent protein synthesis in cells
  • OPP and 5 FAM-Azide used to detect nascent proteins by flow cytometry, microscopy, or plate reader
  • Sufficient reagents to run up to 100 samples
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Protein Synthesis Assay Kit

Item No. 601100

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $395

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    Product Description

    Cayman’s Protein Synthesis Assay Kit is a non-radioactive method for measuring protein synthesis that employs the cell-permeable, alkyne-containing, puromycin analog O-Propargyl-puromycin (OPP). Upon application to cells, the OPP probe incorporates into the C-terminus of translating polypeptide chains, thereby stopping translation. These truncated C-terminal alkyne-labeled proteins are then subsequently detected via copper-catalyzed click chemistry using 5 FAM-Azide. The reagents provided in the kit are sufficient to run 20 samples when using flow cytometry or 100 samples when using a 96-well plate format.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Della Volpe, L., Lee, A.J., Antoszewski, M., et al. Inhibiting ferroptosis enhances ex vivo expansion of human haematopoietic stem cells. Nat. Cell Biol. 27(12), 2214-2224 (2025).

    Chen, Y.-T., Chen, J.-J., and Wang, H.-T. Targeting RNA polymerase I with hernandonine inhibits ribosomal RNA synthesis and tumor cell growth. Mol. Cancer Res. 17(11), 2294-2305 (2019).

    Shen, P., Reineke, L.C., Knutsen, E., et al. Metformin blocks MYC protein synthesis in colorectal cancer via mTOR-4EBP-eIF4E and MNK1-eIF4E signaing. Mol. Oncol. 12, 1856-1870 (2018).