For the induction of a respiratory burst response in neutrophils and monocytes, and quantification by flow cytometry.
Features
  • Samples include whole blood from any species or cells of any type that are capable of producing a NADPH oxidase-dependent respiratory burst
  • Induce and quantify a respiratory burst response
  • Analyze by flow cytometry
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Neutrophil/Monocyte Respiratory Burst Assay Kit

Item No. 601130

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea

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    Product Description

    Respiratory burst, or the rapid generation of reactive oxygen species from immune cells, is crucial for the destruction of invading microorganisms by phagocytes. During this process, professional phagocytes convert molecular oxygen to superoxide anions through the action of NADPH oxidase, which is then converted to reactive oxygen species with potent antimicrobial activity. Cayman's Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.