A cell-based assay for studying modulators of cellular oxidized LDL uptake
Features
  • Detect oxidized LDL uptake by cultured cells by flow cytometry or fluorescence microscopy
  • Flow-based readout allows possibility of multiplexing
  • Inhibitor of oxLDL uptake is included as a control as well as 7-AAD for live-cell gating
  • Sufficient reagents for up to 48 samples
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Oxidized LDL Uptake Assay Kit

Item No. 601180

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • oxLDL
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea

    $472

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    Product Description

    The oxidation of low density lipoprotein is thought to be one of the first steps in atherosclerosis. The uptake of oxidized LDL (oxLDL) by macrophages activates pro-inflammatory mediators that promote disease progression. Cayman’s Oxidized LDL Uptake Assay Kit is a convenient tool to study modulators of cellular uptake of oxLDL. The kit employs a fluorescently tagged, human oxLDL along with myricetin, an inhibitor of uptake that reduces CD36 expression.1 The reagents provided in this kit are sufficient to run a single plate of cultured cells by either flow cytometry or fluorescence microscopy.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lian, T.-W., Wang, L., Lo, Y.-H., et al. Fisetin, morin and myricetin attenuate CD36 expression and oxLDL uptake in U937-derived macrophages. Biochim. Biophys. Acta 1781(10), 601-609 (2008).

    Product Citations

    Cho, D.I., Ahn, M.J., Cho, H.H., et al. ANGPTL4 stabilizes atherosclerotic plaques and modulates the phenotypic transition of vascular smooth muscle cells through KLF4 downregulation. Exp. Mol. Med. 55(2), 426-442 (2023).

    Balamurugan, K., Medishetti, R., Kotha, J., et al. PHLPP1 promotes neutral lipid accumulation through AMPK/ChREBP-dependent lipid uptake and fatty acid synthesis pathways. iScience 25, 103766 (2022).

    Xu, S., Chaudhary, O., Rodriguez-Morales, P., et al. Uptake of oxidized lipids by the scavenger receptor CD36 promotes lipid peroxidation and dysfunction in CD8+ T cells in tumors. Immunity 54, 1561-1577 (2021).

    Wang, R., Lohray, R., Chow, E., et al. Selective uptake of carboxylated multi-walled carbon nanotubes by class A type 1 scavenger receptors and impaired phagocytosis in alveolar macrophages. Nanomaterials (Basel) 10(12), 2417 (2020).