EP₄ Receptor (human) Reporter Assay Kit

Prostaglandin E₂ (PGE₂) is one of the most important biologically active prostanoids which exerts its actions mainly by binding to four distinct E-type prostanoid receptors: EP₁, EP₂, EP₃, and EP₄. These four G protein-coupled receptors (GPCRs) exhibit differences in signal transduction mechanisms, tissue localization, and regulation of expression. EP₄ receptors are highly expressed in the intestine, but are found in lower levels in the lung, kidney, thymus, uterus, and brain. The receptors are primarily coupled to G_s to stimulate the cAMP second messenger signal transduction pathway. EP₄ receptors play important roles in relaxation of smooth muscle, gastric acid secretion, intestinal epithelial transportation, adrenal aldosterone secretion, uterine functions, bone formation, bone resorption, immunity, and inflammation. They have been suggested to involve in colorectal carcinogenesis, neuroprotection in excitotoxic brain injury, and cardiac hypertrophy. The diverse effects of PGE₂ via EP₄ receptors point to the need to identify novel agonists, antagonists, and modulators, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.