A mass spectrometry-based, ratiometric assay for hydrogen peroxide detection in mitochondria
Features
  • An easy-to-use ratiometric assay for hydrogen peroxide detection in mitochondria
  • Measure H2O2 in vivo, in tissues, or in cell culture
  • Designed for use in LC/MS based applications
  • Includes all necessary standards and internal standards provided at known concentrations
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Hydrogen Peroxide Ratiometric MaxSpec® Kit

Item No. 601460

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • H2O2 Ratiometric MaxSpec® Kit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    1 ea

    $255

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    Product Description

    Cayman’s Hydrogen Peroxide Ratiometric MaxSpec® Kit enables the measurement of mitochondrial hydrogen peroxide (H2O2) in cell culture and animal models. MitoB (3-hydroxybenzyl)triphenylphosphonium bromide or MitoBoronic acid) is taken up specifically by polarized mitochondria and accumulates in the matrix. The selective reaction of the arylboronic acid moiety of MitoB with H2O2 forms the phenol product, MitoP (MitoPhenol). Therefore, the increase in the MitoP:MitoB ratio over time indicates the level of mitochondrial H2O2. The ratio of MitoP:MitoB is measured in sample extracts by LC-MS/MS using deuterated internal standards (MitoB-d15 and MitoP-d15) to correct for extraction and detection variations.

    WARNING This product is not for human or veterinary use.