Prostaglandin F Receptor Reporter Assay Kit

Prostaglandin F_2α (PGF_2α) is a biologically important prostanoid that exerts its actions through binding to the PGF_2α receptor (FP).¹ PGF_2α is synthesized in many tissues, including the eye and organs of the female reproductive system. Activation of ocular FP receptors via agonists such as bimatoprost and travoprost reduces ocular hypertension.^2,3 Antagonism of the FP receptor inhibits uterine contraction and delays parturition in rodent models, while knockout of the receptor inhibits parturition in mice, an effect that can be restored through ovariectomy during pregnancy.^4,5 FP receptor antagonism also decreases endometriosis-like lesions and reduces cell proliferation and angiogenesis in a nude mouse endometrial xenograft model.⁶ In addition, FP receptor knockout increases neurological deficits and lesion volume in mice following intracerebral hemorrhagic stroke.⁷ These findings highlight the importance of the FP receptor in reproductive and neurological disorders and emphasize the need for continued research and drug discovery in these areas.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

1. Hata, A.N., and Breyer, R.M. Pharmacology and signaling of prostaglandin receptors: Multiple roles in inflammation and immune modulation. Pharmacol. Ther. 103(2), 147-166 (2004).

2. Maxey, K.M., Johnson, J., and LaBrecque, J. The hydrolysis of bimatoprost in corneal tissue generates a potent prostanoid FP receptor agonist. Surv. Ophthalmol. 47(Suppl. 1), S34-S40 (2002).

3. Sorbera, L.A., and Castañer, J. Travoprost. Drugs Future 25(1), 41-45 (2000).

4. Pohl, O., Chollet, A., Kim, S.H., et al. OBE022, an oral and selective prostaglandin F2a receptor antagonist as an effective and safe modality for the treatment of preterm labor. J. Pharm. Exp. Ther. 366(2), 349-364 (2018).

5. Sugimoto, Y., Yamasaki, A., Segi, E., et al. Failure of parturition in mice lacking the prostaglandin F receptor. Science 277, 681-683 (1997).

6. Ahmad, S.F., Akoum, A., and Horne, A.W. Selective modulation of the prostaglandin F2a pathway markedly impacts on endometriosis progression in a xenograft mouse model. Mol. Hum. Reprod. 21(12), 905-916 (2015).

7. Mohan, S., Koller, E.J., Fazal, J.A., et al. Genetic deletion of PGF2a-FP receptor exacebates brain injury following experimental intracerebral hemorrhage. Front. Neurosci. 12, 556 (2018).