A fluorometric assay for the detection of MPO peroxidation activity in cell lysates and purified enzyme preparations
Features
  • Measure MPO peroxidase activity in cell lysates and purified preparations
  • Assay 38 samples in duplicate
  • Plate-based fluorometric measurement (ex 530-540 nm, em 585-595 nm)
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Myeloperoxidase Peroxidation Fluorometric Assay Kit

Item No. 700160

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • MPO Peroxidation Assay Kit
Emission
590 nm
Excitation
530 nm
Assay Range
0-1.5 µM
Sensitivity
0.08 U/ml
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    2 x 96 wells

    $280

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    Product Description

    Cayman’s MPO Peroxidation Fluorometric Assay provides a convenient fluorescence-based method for detecting MPO peroxidase activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the peroxidase component of MPO, where a single two-electron oxidation of native enzyme (MPO) to compound I (MPO-I) is followed by two successive one-electron reductions back to native enzyme by compound II (MPO-II).1 The reaction between hydrogen peroxide and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin. Resorufin fluorescence can be easily analyzed with an excitation wavelength of 530 nm and emission wavelength of 590 nm. The kit includes an MPO-specific inhibitor for distinguishing MPO activity from MPO-independent fluorescence.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Baldus, S., Heeschen, C., Meinertz, T., et al. Myeloperoxidase serum levels predict risk in patients with acute coronary syndromes. Circulation 108(12), 1440-1445 (2003).