A convenient fluorescence-based method for screening SIRT2 inhibitors or activators
Features
  • Screen for modulators of SIRT2 activity
  • Assay 45 samples in duplicate
  • Plate-based fluorometric measurement (ex 340-360 nm, em 450-465 nm)
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SIRT2 Direct Fluorescent Screening Assay Kit

Item No. 700280

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Emission
465 nm
Excitation
360 nm
License
Sold under license from Cyclex.
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $383

    Add
    Cayman Chemical
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    Product Description

    Cayman’s SIRT2 Direct Screening Assay Kit provides a convenient fluorometric method for screening SIRT2 modulators in a two-step reaction. In the first step, the SIRT2/SIRT3 peptide and NAD+ are incubated with human recombinant SIRT2. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product. The fluorophore can be easily analyzed using an excitation wavelength of 360 nm and an emission wavelength of 465 nm.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Damonte, P., Sociali, G., Parenti, M.D., et al. SIRT6 inhibitors with salicylate-like structure show immunosuppressive and chemosensitizing effects. Bioorg. Med. Chem. 25(20), 5849-5858 (2017).