A simple and sensitive tool for assaying glycogen from tissue
Features
  • Measure glycogen in tissue/cells using fluorometric or colorimetric read-out
  • Assay 20 samples in duplicate
  • Assay range is 0.003-0.2 µg for the fluorometric assay and 0.031-2 µg for the colorimetric assay
  • Plate-based fluorometric measurement (ex 530-540 nm, em 585-595 nm) or colorimetric measurement at 570 nm
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Glycogen Assay Kit

Item No. 700480

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Assay Range
0.2-0.003 µg (fluorometric); 2-0.031 µg (colorimetric)
Lower Limit of Quantification
0.003 µg (fluorometric); 0.031 µg (colorimetric)
Origin
Plant/Armoracia rusticana
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $494

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    Product Description

    Cayman’s Glycogen Assay provides a simple, reproducible, and sensitive tool for measuring glycogen in tissue/cells. Glycogen is hydrolyzed by amyloglucosidase to form β-D-glucose, which is then specifically oxidized by glucose oxidase to form hydrogen peroxide. Hydrogen peroxide, in the presence of horseradish peroxidase, reacts with 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) in a 1:1 stoichiometry to generate the highly fluorescent product resorufin. Resorufin fluorescence is measured with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm. Alternatively, the absorbance can be measured at 570 nm.

    This assay offers the option to measure absorbance or fluorescence. It is at the user’s discretion to choose the mode of measurement (and corresponding standard curve preparation) that best fits their needs. When read fluorometrically, this assay has a dynamic range of 0.2-0.003 µg and a lower limit of quantification (LLOQ) of 0.003 µg. When read colorimetrically, the dynamic range is 2-0.031 µg, with an LLOQ of 0.031 µg.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Maatta, J., Sissala, N., Dimova, E.Y., et al. Hypoxia causes reductions in birth weight by altering maternal glucose and lipid metabolism. Sci. Rep. 8(13583), (2018).

    Liu, Z., Cordoba-Chacon, J., Kineman, R.D., et al. Growth hormone control of hepatic lipid metabolism. Diabetes 65(12), 3598-3609 (2016).

    Ledee, D.R., Kajimoto, M., Priddy, C.M.O., et al. Pyruvate modifies metabolic flux and nutrient sensing during extracorporeal membrane oxygenation in an immature swine model. Am. J. Physiol. Heart Circ. Physiol. 309, H137-H146 (2015).

    Tavecchio, M., Lisanti, S., Bennett, M.J., et al. Deletion of cyclophilin D impairs b-oxidation and promotes glucose metabolism. Sci. Rep. 5, 15981 (2015).