For the accurate measurement of total hemoglobin concentration in various samples
Features
  • Measure total hemoglobin concentration in blood, tissue homogenates, and cell lysates
  • Assay 40 samples in duplicate
  • Assay Range: 0.016-0.4 g/dl
  • Plate-based colorimetric measurement (560-590 nm)
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Hemoglobin Colorimetric Assay Kit

Item No. 700540

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Hb
  • Hgb
Limit of Detection
3 µM (0.005 g/dl)
Shipping & Storage Information
Storage
4°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    2 x 96 wells

    $347

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    Product Description

    Hemoglobin (Hb or Hgb) is a globular protein found primarily in erythrocytes that carries oxygen from the lungs to tissue where it releases oxygen and then returns carbon dioxide (CO2) from the tissue to the lungs. Beyond binding and transporting oxygen, hemoglobin also binds CO2, carbon monoxide (CO), and nitric oxide (NO). Cayman’s Hemoglobin Colorimetric Assay provides a quick, reliable method for determining total hemoglobin concentration in a variety of biological samples, including blood, tissue homogenates, and cell lysates. Unlike the universally accepted reference method of hemoglobin determination, which uses potassium cyanide as a reagent and commonly under-estimates hemoglobin levels, Cayman’s Hemoglobin Assay utilizes an optimized detection reagent which is non-toxic, reporting accurate measurements of total hemoglobin concentrations with an absorbance between 560-590 nm.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Pang, Y.-L., Fang, S.-Y., Cheng, T.-T., et al. Viable allogeneic mitochondria transplantation improves gas exchange and alveolar-capillary permeability in rats with endotoxin-induced acute lung injuries. Int. J. Med. Sci. 19(6), 1036-1046 (2022).

    Wood, J., Yasmin-Karim, S., Moreau, M., et al. Characterization of isolated extracts from Justicia plant leaves used as remedy for anemia. Molecules 25(3), 534 (2020).

    Liu, M., Huang, M., Huang, L., et al. New perspectives on Galleria mellonella larvae as a host model using Riemerella anatipestifer as a proof of concept. Infect. Immun. 87(8), e00072-00019 (2019).

    Salas-Perdomo, A., Miró-Mur, F., Gallizioli, M., et al. Role of the S1P pathway and inhibition by fingolimod in preventing hemorrhagic transformation after stroke. Sci. Rep. 9(1), 8309 (2019).

    Zhang, X., Bohner, A., Bhuvanagiri, S., et al. Targeted intraceptor nanoparticle for neovascular macular degeneration: Preclinical dose optimization and toxicology assessment. Mol. Ther. 25(7), 1606-1615 (2017).

    Greene, K.S., Choi, A., Yang, N., et al. Glutamine metabolism is essential for coronavirus replication in host cells and in mice. The Journal of Biological Chemisty 301(1), 108063 (2025).