A convenient fluorometric method of screening human PAD4 inhibitors
Features
  • Screen for inhibitors of PAD4
  • Assay 45 samples in duplicate
  • Plate-based fluorometric measurement (ex 405-415 nm, em 470-480 nm)
  • Orthogonal assay to Cayman’s PAD4 Inhibitor Screening Assay Kit (AMC) (Item No. 701320)
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PAD4 Inhibitor Screening Assay Kit (Ammonia)

Item No. 700560

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • PADI4
  • PADI5
  • PDI5
  • Peptidylarginine Deiminase 4
  • Protein Arginine Deiminase 4
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $569

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    Product Description

    Cayman’s PAD4 Inhibitor Screening Assay Kit (Ammonia) provides a convenient method for screening human PAD4 inhibitors. PAD4 deiminates N-α-benzoyl-L-arginine ethyl ester (BAEE), a non-natural substrate with similar kinetic properties to the natural substrates, producing ammonia.3 Ammonia reacts with a detector resulting in a fluorescent product. Fluorescence is then analyzed with an excitation wavelength of 405-415 nm and an emission wavelength of 470-480 nm.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Tao, L., Xu, M., Dai, X., et al. Polypharmacological profiles underlying the antitumor property of Salvia miltiorrhiza root (Danshen) interfering with NOX-dependent neutriphil extracellular traps. Oxid. Med. Cell. Longev. 4908328, (2018).