For the detection of urea in plasma, serum, and urine
Features
  • Measure urea in plasma, serum, and urine
  • Assay 20 samples in duplicate
  • Assay Range: 50 μM-1 mM
  • Plate-based fluorometric measurement (ex 405-415 nm, em 470-480 nm)
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Urea Fluorometric Assay Kit

Item No. 700620

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Origin
Plant/Canavalia ensiformis
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $398

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    Product Description

    Urea or carbamide is synthesized in the body of many organisms as part of the urea cycle. It is the final degradation product of protein and amino acid metabolism. Urea is found in blood and is excreted by the kidney as a component of urine. Besides its role as carrier of waste nitrogen, urea also plays a role in the countercurrent exchange system of the nephrons, allowing for re-absorption of water and critical ions from the excreted urine. Cayman’s Urea Assay provides a convenient method for detecting urea in plasma, serum, and urine. In this assay, urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Ammonia reacts with the detector resulting in a fluorescent product. Fluorescence is analyzed with an excitation wavelength of 405-415 nm and an emission wavelength of 470-480 nm.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Wang, A., Huen, S.C., Luan, H.H., et al. Opposing effects of fasting metabolism on tissue tolerance in bacterial and viral inflammation. Cell 166(6), 1512-1525 (2016).

    James, L.R., Le, C., Doherty, H., et al. Connective tissue growth factor (CTGF) expression modulates response to high glucose. PLoS One 8(8), e70441 (2013).