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TBARS (TCA Method) Assay Kit

Item № 700870
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     96 wells $149.00 0.00

Pricing updated 2019-06-16. Prices are subject to change without notice.

  • Measure TBARS in plasma, serum, urine, tissue homogenates, and cell lysates
  • Assay 40 samples in duplicate
  • Assay range: 0.0625-50 µM
  • Plate-based colorimetric measurement (530-540 nm) or fluorometric measurement (ex 530 nm, em 550 nm)
  • Thiobarbituric Acid Reactive Substances (TCA Method)

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from PUFAs, are unstable and decompose to form a complex series of compounds, which include reactive carbonyl compounds, such as malondialdehyde (MDA). MDA can be quantified through a controlled reaction with thiobarbituric acid, generating 'Thiobarbituric Acid Reactive Substances' (TBARS). Cayman’s TBARS Assay Kit provides a simple, reproducible, and standardized tool for assaying lipid peroxidation in plasma, serum, urine, tissue homogenates, and cell lysates. The MDA-TBA adduct formed by the reaction of MDA and TBA under high temperature (90-100°C) and acidic conditions can be measured either colorimetrically at 530-540 nm or with much higher sensitivity fluorometrically at an excitation wavelength of 530 nm and an emission wavelength of 550 nm.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Thiobarbituric Acid Reactive Substances (TCA Method)

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Citations

Zadeh-Ardabili, P.M., Rad, S.K., Rad, S.K., et al. Palm vitamin E reduces locomotor dysfunction and morphological changes induced by spinal cord injury and protects against oxidative damage Sci. Rep. 7(14365), (2017).

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