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Catalase Assay Kit (without Hydrogen Peroxide)

Item № 700910
product image
     96 wells $270.00 0.00
     480 wells $490.00 0.00

Pricing updated 2019-06-16. Prices are subject to change without notice.

  • Measure catalase activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates
  • Assay 40 samples in duplicate
  • Measure catalase activity down to 2 U/ml
  • Plate-based colorimetric measurement (540 nm)
  • CAT

Catalase (CAT) is a ubiquitous antioxidant enzyme involved in the detoxification of hydrogen peroxide (H2O2), a toxic product of both normal aerobic metabolism and pathogenic ROS production. It catalyzes the conversion of two molecules of H2O2 to molecular oxygen and two molecules of water (catalytic activity). CAT also demonstrates peroxidatic activity, in which low molecular weight alcohols can serve as electron donors. While aliphatic alcohols serve as specific substrates for CAT, other enzymes with peroxidatic activity do not utilize these substrates. Cayman’s Catalase Assay Kit utilizes the peroxidatic function of CAT for determination of enzyme activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates. The method is based on the reaction of the enzyme with methanol in the presence of an optimal concentration of H2O2. The formaldehyde produced is measured colorimetrically with Purpald, a chromogen that specifically forms a bicyclic heterocycle with aldehydes, which upon oxidation changes from colorless to a purple color.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • CAT
  • Animal/Bovine

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Cayman Contract Services

We work with scientists to accelerate their research, drug discovery, and drug development needs through our expertise in the following areas:

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640