A complete system for quantifying complex I inhibition
Features
  • Measure complex I activity in isolated mitochondria
  • Useful for screening compounds for complex I inhibition
  • Suitable for high-throughput screening
  • Plate-based colorimetric measurement (340 nm)
  • MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010) available to aid in mitochondrial tissue isolation
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MitoCheck® Complex I Activity Assay Kit

Item No. 700930

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $512

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    Product Description

    Complex I (NADH dehydrogenase/NADH:ubiquinone oxidoreductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex I catalyzes the 2 electron oxidation of NADH followed by the reduction of ubiquinone (Q) to form ubiquinol (QH2), and ultimately the reduction of the terminal electron acceptor, O2. During the passage of electrons from NADH to Q, the translocation of four protons (H+) from the mitochondrial matrix to the intermembrane space occurs, contributing to the chemiosmotic proton gradient, which is required for oxidative phosphorylation. Cayman's MitoCheck® Complex I Activity Assay allows for the activity of complex I to be determined without the need to isolate mitochondria or pre-incubate with antibodies. The rate of NADH oxidation is measured by a decrease in absorbance at 340 nm and is proportional to the activity of complex I. For the use of this kit with other types of tissue mitochondria, please see Cayman's MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010).

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Zhang, H., Zhang, T.T., Liu, H., et al. Thioredoxin-mediated ROS homeostasis explains natural variation in plant regeneration. Plant Physiol. 176(3), 2231-2250 (2018).

    Hajmousa, G., Vogelaar, P., Brouwer, L.A., et al. The 6-chromanol derivate SUL-109 enables prolonged hypothermic storage of adipose tissue-derived stem cells. Biomaterials 119, 43-52 (2017).

    Lei, S., Sun, R.-z., Wang, D., et al. Increased hepatic fatty acids uptake and oxidation by LRPPRC-driven oxidative phosphorylation reduces blood lipid levels. Front. Physiol. 7, 270 (2016).