A complete system for quantifying complex IV inhibition
Features
  • Measure complex IV activity in isolated mitochondria
  • Useful for screening compounds for complex IV inhibition
  • Suitable for high-throughput screening
  • Plate-based colorimetric measurement (550 nm)
  • MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010) available to aid in mitochondrial tissue isolation
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MitoCheck® Complex IV Activity Assay Kit

Item No. 700990

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Origin
Animal/Bovine, Animal/Horse
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $492

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    • Bioanalysis and compound screening using Cayman or qualified commercial assay kits
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    Product Description

    Complex IV (cytochrome c oxidase) is the terminal electron acceptor in the mitochondrial electron transport chain. Complex IV functions by oxidizing cytochrome c and completing a four-electron reduction of oxygen (O2) to form water. During this process, two H+ are translocated from the mitochondrial matrix to the intermembrane space, contributing the mitochondrial membrane potential required for ATP synthesis. Common inhibitors of complex IV include potassium cyanide, azide, O2 limitation, nitric oxide, and carbon monoxide. Cayman’s MitoCheck® Complex IV Activity Assay is designed to measure the direct oxidation of cytochrome c by complex IV, in an isolated bovine heart mitochondrial system that is supplied within the kit. For the use of this kit with other types of tissue mitochondria, please see Cayman's MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010).

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Lei, S., Sun, R.-z., Wang, D., et al. Increased hepatic fatty acids uptake and oxidation by LRPPRC-driven oxidative phosphorylation reduces blood lipid levels. Front. Physiol. 7, 270 (2016).

    Hajmousa, G., Vogelaar, P., Brouwer, L.A., et al. The 6-chromanol derivate SUL-109 enables prolonged hypothermic storage of adipose tissue-derived stem cells. Biomaterials 119, 43-52 (2017).