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MitoCheck® Complex V Activity Assay Kit

Item № 701000
product image
     96 wells $385.00 0.00

Pricing updated 2019-07-18. Prices are subject to change without notice.

  • Measure complex V activity in isolated mitochondria
  • Useful for screening compounds for complex V inhibition
  • Suitable for high-throughput screening
  • Plate-based colorimetric measurement (340 nm)
  • MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010) available to aid in mitochondrial tissue isolation

The mitochondrial F1FO ATP synthase, commonly referred to as complex V, is a multi-subunit protein that utilizes energy in the form of a proton gradient generated by the electron transport chain, to phosphorylate ADP to form ATP in the presence of Pi. This mechanism, referred to as oxidative phosphorylation, is responsible for ~90% of ATP generation in mammalian cells. Cayman's MitoCheck® Complex V Activity Assay measures the activity of Complex V, in the reverse reaction, as an ATPase in the mitochondrial samples provided. In this assay, ATP is dephosphorylated ADP by complex V. The ADP is then utilized by pyruvate kinase to convert phosphoenolpyruvate into pyruvate with the concomitant generation of ATP. Pyruvate, in the presence of NADH and lactate dehydrogenase, is then reduced to lactate and NAD+. The rate of NADH oxidation is monitored at 340 nm. Isolated bovine heart mitochondria are provided in this kit. Tissue mitochondria can also be acquired using Cayman’s MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010).

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Animal/Bovine

Warning - this product is not for human or veterinary use.

Shipping & Storage
Dry ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Citations

Hajmousa, G., Vogelaar, P., Brouwer, L.A., et al. The 6-chromanol derivate SUL-109 enables prolonged hypothermic storage of adipose tissue-derived stem cells Biomaterials 119, 43-52 (2017).

Lei, S., Sun, R.-z., Wang, D., et al. Increased hepatic fatty acids uptake and oxidation by LRPPRC-driven oxidative phosphorylation reduces blood lipid levels Front. Physiol. 7, 270 (2016).

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