MitoCheck® Mitochondrial (Tissue) Isolation Kit | Cayman Chemical
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MitoCheck® Mitochondrial (Tissue) Isolation Kit

Item № 701010
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     1 ea $175.00 0.00

Pricing updated 2018-11-16. Prices are subject to change without notice.

Description
Features
  • Isolate coupled mitochondria from fresh tissue samples
  • Isolated mitochondria can be used with MitoCheck® Complex I-V Activity Assay Kits and Citrate Synthase Activity Assay Kit

When measuring mitochondrial function or the effects of an unknown on mitochondrial function, it can be difficult to determine a mechanism using whole organisms or tissue. When this is the case, isolated mitochondria provide a simple and biochemically relevant experimental model. Cayman’s MitoCheck® Mitochondrial (Tissue) Isolation Kit includes a complete package of reagents and step-by-step instructions for isolating coupled mitochondria* from freshly harvested mammalian heart, liver, or kidney tissue through a process of differential centrifugation in isotonic buffers. *Coupled mitochondria are defined as mitochondria capable of phosphorylating ADP at the F1FO-ATP synthase (Complex V) through the utilization of a proton (H+) gradient, which is generated by the electron transport chain (ETC) in the presence of appropriate substrates (e.g., succinate) and in the absence of uncouplers and mitochondrial inhibitors.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 6 months
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

Technical Support
Contact Us
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  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of MitoCheck® Mitochondrial (Tissue) Isolation Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640