For quantifying citrate synthase activity in isolated mitochondria, tissue, or cell homogenates
Features
  • Measure citrate synthase activity in isolated mitochondria, tissue, or cell homogenate
  • Assess mitochondrial content
  • Suitable for high-throughput screening
  • Plate-based colorimetric measurement (412 nm)
  • MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010) available to aid in mitochondrial tissue isolation
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MitoCheck® Citrate Synthase Activity Assay Kit

Item No. 701040

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Origin
Animal/Pig
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $480

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    Product Description

    The condensation of the dicarboxylate oxaloacetate and acetyl CoA to the tricarboxylate citrate is catalyzed by citrate synthase. It is within this reaction that carbon molecules (as acetyl CoA) obtained from pyruvate oxidation are fed into the tricarboxylic acid (TCA or citric acid) cycle. As a mitochondrial enzyme, citrate synthase is commonly used as a normalization factor for mitochondrial protein, but can also be used as a biomarker for mitochondrial content in a tissue homogenate. Cayman's MitoCheck® Citrate Synthase Activity Assay Kit allows for the simple and convenient determination of citrate synthase activity from isolated mitochondria, tissue, or cell homogenates. This assay measures the production of SH-CoA by monitoring the absorbance of Citrate Synthase Developing Reagent at 412 nm in a convenient 96-well format.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Marottoli, F.M., Trevino, T.N., Geng, X., et al. Autocrine effects of brain endothelial cell-produced human apolipoprotein E on metabolism and inflammation in vitro. Front. Cell Dev. Biol. 9, (2021).

    Lei, S., Sun, R.-z., Wang, D., et al. Increased hepatic fatty acids uptake and oxidation by LRPPRC-driven oxidative phosphorylation reduces blood lipid levels. Front. Physiol. 7, 270 (2016).