A colorimetric assay for the screening of isozyme-specific inhibitors
Features
  • Screen for inhibitors of ovine COX-1 or human COX-2
  • Measure the peroxidase component of the COX
  • Assay 41 samples for COX-1 and 41 samples for COX-2 in duplicate
  • Plate-based colorimetric measurement (590 nm)
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COX Colorimetric Inhibitor Screening Assay Kit

Item No. 701050

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Origin
Animal/Sheep
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    2 x 96 wells

    $908

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    Product Description

    The COX Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) at 590 nm.1 Inhibition of COX activity, measured by TMPD oxidation, by a variety of selective and nonselective inhibitors, shows changed potencies similar to those observed with other in vitro methods. The COX Colorimetric Inhibitor Screening assay includes both ovine COX-1 and human recombinant COX-2 enzymes in order to screen isozyme-specific inhibitors. The Cayman COX Colorimetric Assay is a time saving tool for screening vast numbers of inhibitors.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kulmacz, R.J., and Lands, W.E.M. Requirements for hydroperoxide by the cyclooxygenase and peroxidase activities of prostaglandin H synthase. Prostaglandins 25(4), 531-540 (1983).

    Product Citations

    Romero-Estrada, A., Maldonado-Magaña, A., González-Christen, J., et al. Anti-inflammatory and antioxidative effects of six pentacyclic triterpenes isolated from the Mexican copal resin of Bursera copallifera. BMC Complement. Altern. Med. 16(1), 422 (2016).