A convenient method of screening human COX-1 and COX-2 inhibitors
Features
  • Screen for inhibitors of human COX-1 or COX-2
  • Directly measure PGF2α produced in the COX reaction
  • Assay 15 samples for COX-1 or COX-2 in duplicate
  • Plate-based colorimetric measurement (405-420 nm)
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COX (human) Inhibitor Screening Assay Kit

Item No. 701230

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Cyclooxygenase
  • PGHS
  • Prostaglandin H Synthase
Assay Range
3.9-500 pg/ml
Sensitivity
10 pg/ml
Cross Reactivity
(100%) Prostaglandin F2α(244.1%) Prostaglandin F2α Ethanolamide(61%) Prostaglandin F1α(21%) Prostaglandin F3α(5.3%) 20-hydroxy Prostaglandin F2α(2.6%) Prostaglandin E2(1.2%) Prostaglandin D2(0.69%) 19(R)-hydroxy Prostaglandin F2α(0.1%) 13,14-dihydro-15-keto Prostaglandin F2α(0.03%) 11β-Prostaglandin F2α(0.02%) 6,15-diketo-13,14-dihydro Prostaglandin F1α(0.01%) 11-dehydro Thromboxane B(<0.01%) 8-iso Prostaglandin F2α
Origin
Animal/Bovine, Animal/Eel, Animal/Rabbit
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $480

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    Product Description

    The COX (human) Inhibitor Screening Assay directly measures PGF2α by stannous chloride reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via ELISA using an antiserum specific to PGF2a.

    This assay includes recombinant human COX-1 and COX-2 and is an excellent tool for general COX-1 and COX-2 inhibitor screening. It will also help to eliminate false positive leads generated by less specific methods.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Fan, X., Li, J., Deng, X., et al. Design, synthesis and bioactivity study of N-salicyloyl tryptamine derivatives as multifunctional agents for the treatment of neuroinflammation. Eur. J. Med. Chem. 193, 112217 (2020).

    Parashar, B., Shankar, S.L., O'Guin, K., et al. Inhibition of human neuroblastoma cell growth by CAY10404, a highly selective Cox-2 inhibitor. J. Neurooncol. 71(2), 141-148 (2005).