A colorimetric or fluorometric assay for the quantification of α-KG in serum, plasma, urine, and tissue
Features
  • Measure α-ketoglutarate in plasma, serum, urine, and tissue
  • Assay 24 samples in duplicate or 18 in triplicate
  • Assay range: 10-200 µM with fluorometric detection and 50-1,000 µM with colorimetric detection
  • Plate-based fluorometric measurement (ex. 530-540 nm, em. 585-595 nm) or colorimetric measurement
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α-Ketoglutarate Detection Assay Kit

Item No. 701350

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • α-KG
  • 2-Oxoglutarate
Limit of Detection
1 µM (0.01 nmol/well) (fluorometric); 13 µM (0.13 nmol/well) (colorimetric)
Lower Limit of Quantification
10 µM (0.1 nmol/well) (fluorometric); 50 µM (0.5 nmol/well) (colorimetric)
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $468

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    Product Description

    Cayman’s α-KG Detection Assay provides fluorometric- or colorimetric-based methods for quantifying α-KG in biological samples such as serum, plasma, urine, and tissue. It can also be utilized to determine intracellular and extracellular α-KG concentrations in cell culture samples. In this assay, alanine transaminase (ALT) deaminates α-KG to form pyruvate.1,2 Pyruvate is then oxidized by pyruvate oxidase to yield acetyl phosphate, hydrogen peroxide (H2O2), and carbon dioxide.3 As H2O2 is produced, the cycling mechanism of horseradish peroxidase (HRP) results in the concurrent reduction of H2O2 to H2O and the oxidation of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) to produce the highly fluorescent compound resorufin.4 Resorufin fluorescence is analyzed with an excitation wavelength between 530-540 nm and an emission wavelength between 585-595 nm. Alternatively, the absorbance of resorufin can be measured at 570 nm.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. DeRosa, G., and Swick, R.W. Metabolic implications of the distribution of the alanine aminotransferase isoenzymes. The Journal of Biological Chemisty 250(20), 7961-7967 (1975).

    2. Krebs, H.A. Metabolism of amino-acids. Deamination of amino-acids. Biochem. J. 29(7), 1620-1644 (1935).

    3. Lindsay, M., O'Donnell, T.V., and Edson, N.L. The oxidation of pyruvate and fatty acids by Mycobacterium ranae. Biochem J. 46(2), 248-257 (1950).

    4. Zhou, M., Diwu, Z., Panchuck-Voloshina, N., et al. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal. Biochem. 253(2), 162-168 (1997).