Measure mitochondrial ROS levels in living cells
Features
  • Measures mitochondrial ROS directly in living cells
  • Includes antimycin A as a positive control
  • Flow cytometry, fluorescence, and plate-based methods
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Mitochondrial ROS Detection Assay Kit

Item No. 701600

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Mitochondrial Reactive Oxygen Species Detection Assay Kit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $275

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    Product Description

    As one of the major sources of reactive oxygen species (ROS) in the cell, mitochondrial ROS has often been difficult to single out from the other sources of ROS within the cell. While some ROS dyes such as DHE and DCFDA tend to be less location-specific and dyes such as ADHP are only extracellular, Mitochondrial ROS Detection Reagent is targeted primarily to the mitochondrion making Mitochondrial ROS Detection Reagent an ideal reagent to study mitochondrial ROS.1,2,3

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Michalski, R., Michalowski, B., Sikora, A., et al. On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: A reassessment. Free Redic. Biol. Med. 67, 278-284 (2014).

    2. Hoffmann, A., Bächner, D., Betat, N., et al. Developmental expression of murine β-trace in embryos and adult animals suggests a function in maturation and maintenance of blood-tissue barriers. Dev. Dyn. 207, 332-343 (1996).

    3. Zielonka, J., Zielonka, M., Sikora, A., et al. Global profiling of reactive oxygen and nitrogen species in biological systems. The Journal of Biological Chemisty 287(5), 2984-2995 (2012).

    Product Citations

    Wang, X., Shelton, S.D., Bordieanu, B., et al. Scinderin promotes fusion of electron transport chain dysfunctional muscle stem cells with myofibers. Nat. Aging 2(2), s43587-43021-00164-x. (2024).

    Rizk, F.H., El Saadany, A.A., Atef, M.M., et al. Ulinastatin ameliorated streptozotocin-induced diabetic nephropathy: Potential effects via modulating the components of gut-kidney axis and restoring mitochondrial homeostasis. Pflugers Arch. 475(10), 1161-1176 (2023).

    Grossini, E., Vebkatesan, S., Alkabes, M., et al. Membrane blue dual protects retinal pigment epithelium cells/ganglion cells-like through modulation of mitochondria function. Biomedicines 10(11), 2854 (2022).