A convenient fluorometric method of detecting ENPP1 and/or ENPP3 activity in cells
Features
  • A convenient assay to check the activities of ENPP1/ENPP3 in live cells
  • Assay 22 samples in duplicate or 14 samples in triplicate
  • Plate-based fluorometric measurement with an excitation and emission wavelengths of 485 and 520 nm, respectively
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ENPP1/ENPP3 Cell-Based Activity Assay Kit

Item No. 702080

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Ectonucleotide Pyrophosphatase/Phosphodiesterase I
  • Ectonucleotide Pyrophosphatase/Phosphodiesterase III
  • Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1
  • Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 3
Emission
520 nm
Excitation
485 nm
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $471

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    Cayman Chemical
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    Product Description

    Cayman’s ENPP1/ENPP3 Cell-Based Activity Assay Kit provides a robust and easy-to-use platform for determining the activities of ENPP1 and/or ENPP3 in live cells. The assay uses an ENPP1/ENPP3-specific fluorogenic substrate, TG-mAMP.1 ENPP1/ENPP3 cleaves this substrate generating free Tokyo Green™, which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 485 and 520 nm, respectively. Respective ENPP1 and ENPP3 activities can be distinguished from one another by the use of specific ENPP1 inhibitor, ENPP1 Inhibitor C or the ENPP1/ENPP3 Dual Inhibitor Mix provided in this kit.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kawaguchi, M., Han, X., Hisada, T., et al. Development of an ENPP1 fluorescence probe for inhibitor screening, cellular imaging, and prognostic assessment of malignant breast cancer. J. Med. Chem. 62(20), 9254-9269 (2019).

    Product Citations

    Ji, H., Jiang, W., Zhang, J., et al. ENPP1 governs the metabolic regulation of effector T cells in autoimmunity by detecting cytosolic mitochondrial DNA. Cell Rep. 44(6), 115851 (2025).