15-hydroxy Prostaglandin Dehydrogenase Inhibitor Screening Kit

Cayman’s 15-PGDH Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human 15-PGDH, the enzyme that catalyzes the oxidation of prostaglandins to 15-keto metabolites with reduced biological activity. Measurement of 15-PGDH activity is carried out by monitoring the reduction of NAD⁺ to NADH. The reduction of NAD⁺ to NADH is accompanied by an increase in fluorescence at 445 nm following excitation at 340 nm. The potent and reversible 15-PGDH inhibitor ML-148 Assay Reagent is included as a positive control and inhibits 15-PGDH with an IC₅₀ value of 11 nM.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.