sPLA₂ (Type IIA) Inhibitor Screening Assay Kit

Cayman’s sPLA₂ (Type IIA) Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human sPLA₂ type IIA, a phospholipase that preferentially hydrolyzes PG and PE. The assay uses diheptanoyl thio-PE, a sulfur-containing analog of PE as a substrate. Upon hydrolysis by sPLA₂ type IIA at the sn-2 position, the free thiol can be detected by the sPLA₂ Assay Probe. This results in a product that can be easily quantified by measuring absorbance at a wavelength of 410 nm. A potent and reversible inhibitor, LY315920 (included in this kit as sPLA₂ Control Inhibitor), inhibits the enzyme with an average IC₅₀ value of approximately 22 nM.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.