A convenient colorimetric method of screening human sPLA2 Type X inhibitors
Features
  • Screen for inhibitors of human sPLA2 (Type X)
  • Plate-based colorimetric measurement (410 nm)
  • Includes recombinant human sPLA2 (Type X) and a positive control inhibitor
  • Includes assay plate
  • Assay up to 125 test inhibitors in triplicate or up to 93 test inhibitors in quadruplicate
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sPLA2 (Type X) Inhibitor Screening Assay Kit

Item No. 702480

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • GXsPLA2
  • Phosphatidylcholine 2-Acylhydrolase 10
  • PLA2G10
  • Secretory Phospholipase A2 (Group 10)
  • Secretory Phospholipase A2 (Group X)
  • Secretory Phospholipase A2 (mouse Group X)
  • Secretory Phospholipase A2 (Type X)
  • sPLA2-X
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    384 wells

    $602

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    Product Description

    Cayman’s sPLA2 (Type X) Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human sPLA2 (Type X), a phospholipase that hydrolyzes PE and PC at the sn-2 position. The assay uses a sulfur-containing analog of 1,2-diheptanoyl-sn-glycero-3-PC (di-C7-PC) as a substrate. Upon hydrolysis by sPLA2 (Type X) at the sn-2 position, free thiols can be detected by the sPLA2 Assay Probe resulting in a product that can be easily quantified using its absorbance at a wavelength of 410 nm. The potent and reversible sPLA2 (Type X) inhibitor, sPLA2 Control Inhibitor, also known as LY315920 or varespladib, is included as a positive control and inhibits the enzyme with an IC50 value of approximately 11 nM.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.