A convenient colorimetric method of screening human sPLA2 Type V inhibitors
Features
  • Screen for up to 125 potential inhibitors of human sPLA2 (Type V) in triplicate or up to 93 in quadruplicate
  • Plate-based colorimetric measurement (410 nm)
  • Includes sPLA2 (Type V) and a positive control inhibitor
  • Assay plate included
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sPLA2 (Type V) Inhibitor Screening Assay Kit

Item No. 702490

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • GVsPLA2
  • Phosphatidylcholine 2-acylhydrolase 5
  • Phospholipase A2 Group V
  • PLA2G5
  • Secretory Phospholipase A2 (Type V)
  • sPLA2-V
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    384 wells

    $549

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    Product Description

    Cayman’s sPLA2 (Type V) Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human sPLA2 type V. The assay uses diheptanoyl thio-PG, a sulfur-containing analog of phosphatidylglycerol, which is the preferred substrate of sPLA2 type V. Upon hydrolysis by sPLA2 type V at the sn-2 position, the free thiol can be detected by the sPLA2 Assay Probe. This results in a product that can be easily quantified by measuring absorbance at a wavelength of 410 nm. A potent and reversible inhibitor, LY315920 (included in this kit as sPLA2 Control Inhibitor), inhibits the enzyme with an IC50 value of approximately 100 nM.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.