A fluorometric assay for the detection of FHIT activity in cell and tissue lysates
Features
  • A quick and sensitive assay that measures FHIT activity in cell and tissue lysates
  • Convenient 96-well plate, fluorometric readout
  • Active recombinant human FHIT included as a positive control
  • Assay plate included
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FHIT Fluorometric Activity Assay Kit

Item No. 702510

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Ap3Aase
  • Ap3A Hydrolase
  • Bis(5'-adenosyl)-Triphosphatase
  • Diadenosine 5',5"-P1,P3-Triphosphate Hydrolase
  • Dinucleosidetriphosphatase
  • FRA3B
  • Fragile Histidine Triad Protein
Emission
535 nm
Excitation
485 nm
Origin
Bacterium/E. coli
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $545

    Add
    Cayman Chemical
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    Product Description

    Cayman’s FHIT Fluorometric Activity Assay Kit provides a convenient method of detecting FHIT activity in cell and tissue lysates. Measurement of the FHIT activity is carried out by monitoring the hydrolysis of an FHIT substrate analog, TG-1AdaAMP, generating free Tokyo Green™, whose fluorescence can be easily monitored at excitation and emission wavelengths of 485 and 535 nm, respectively.1 The rate of increase in fluorescence is directly proportional to the levels of active FHIT in the sample.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kawaguchi, M., Sekimoto, E., Ohta, Y., et al. Synthesis of fluorescent probes targeting tumor-suppressor protein FHIT and Identification of apoptosis-inducing FHIT inhibitors. J. Med. Chem. 64(13), 9567-9576 (2021).