Resazurin Cell Viability Assay Kit

Cayman’s Resazurin Cell Viability Assay Kit provides a non-toxic and easy-to-use method for analyzing cell viability. Resazurin is a redox-sensitive fluorophore that can be irreversibly reduced by the mitochondrial oxidoreductase enzymes in metabolically active cells to produce resorufin.^1,2,3 Resorufin’s fluorescence can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 560 and 590 nm, respectively. Non-viable cells lack the ability to reduce resazurin to resorufin, and therefore cannot generate a fluorescent signal. The fluorescent signal generated in the assay is proportional to the number of viable cells present.^1,2,3

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

1. Gonzalez, R.J., and Tarloff, J.B. Evaluation of hepatic subcellular fractions for Alamar blue and MTT reductase activity. Toxicol. In Vitro 15(3), 257-259 (2001).

2. O'Brien, J., Wilson, I., Orton, T., et al. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem. 267(17), 5421-5426 (2000).

3. Pagé, B., Pagé, M., and Noël, C. A new fluorometric assay for cytotoxicity measurements in vitro. Int. J. Oncol. 3(3), 473-476 (1993).