LipidLaunch™ LNP Apparent pK_a Assay Kit (TNS Method)

Cayman’s LipidLaunch™ LNP Apparent pK_a Assay Kit (TNS Method) provides a robust and easy-to-use platform for testing the apparent/global pK_a of formulated LNPs. The assay uses a fluorogenic probe, 6-(p-toluidino)-2-naphthalenesulfonic acid sodium salt (TNS). This probe fluoresces in association with positively charged membranes, which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 320 and 450 nm, respectively. TNS loses fluorescence as it dissociates, so a titration of the pH in the presence of LNPs results in a curve, with the 50% maximal fluorescence point called the apparent pK_a of the particle.

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He, Y., Pilkington, E.H., Kang, H.J., et al. Morphological changes induced by buffer exchangeduring preparation of mRNA-lipid nanoparticles occur in a stepwise manner as pH is elevated. Mol. Ther. Methods Clin. Dev. 33, 1-12 (2025).

Balcorta, H.V., Mata Corral, M.Y., Gallegos, A., et al. Development of chemical tags for universal lipid nanoparticle visualization and tracking in 2D and 3D imaging. Nano Lett. (2025).