A luminescent assay for the detection of firefly luciferase activity in cultured cells
Features
  • ​A fast, easy to use bioluminescence assay used to measure luciferase activity in cells
  • Assay up to 240 samples in a 96-well plate format or up to 1,920 samples in a 384-well plate format in duplicate.
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Firefly Luciferase Activity Assay Kit

Item No. 702710

Technical Information
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    Cayman’s Firefly Luciferase Activity Assay Kit provides a convenient and sensitive method for detecting firefly luciferase activity in user-supplied cultured mammalian cells, such as those used in luciferase reporter assays. The included assay buffer efficiently lyses cells, enabling the luciferase enzyme to access D-luciferin and ATP present in the Firefly Luciferase Substrate Mix to produce a luminescent signal. The signal intensity is directly proportional to the level of active firefly luciferase present in the sample.

    This kit contains a sufficient volume of reagents to assay 160 samples in triplicate using a 96-well plate format or 640 samples in triplicate using a 384-well plate format.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.