A fluorometric assay for the detection of caspase-3/7 activity in cell lysates
Features
  • Screen for modulators of caspase-3/7 activity
  • Plate-based fluorometric measurement
  • Includes recombinant human caspase-3 assay positive control and staurosporine apoptosis inducer
  • Includes assay plate
  • Test up tp 28 samples in triplicate or 44 samples in duplicate
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Caspase-3/7 Cell-Based Activity Assay Kit

Item No. 702790

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Apopain
  • Apoptotic Protease Mch-3
  • CASP-3/7
  • CMH-1
  • Cysteine Protease CPP32
  • ICE-LAP3
  • ICE-like Apoptotic Protease 3
  • Protein Yama
  • SCA-1
  • SREBP Cleavage Activity 1
Emission
505 nm
Excitation
400 nm
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $385

    Add
    Cayman Chemical
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    Product Description

    Cayman’s Caspase-3/7 Cell-Based Activity Assay Kit provides a convenient method of detecting caspase-3 and caspase-7 activity in cell lysates. Activity is monitored using a caspase-3/7 specific substrate, N-Ac-DEVD-AFC. Cleavage by the active enzyme(s) generates a highly fluorescent product that can be measured using excitation and emission wavelengths of 400 and 505 nm, respectively.Active caspase-3 is included in the kit as a positive control and staurosporine is included to induce apoptosis. The caspase-3/7 inhibitor, N-Ac-DEVD-CHO, is included as a control to confirm the specificity of the fluorescence signal to the activity of the enzymes.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.