Myeloperoxidase Chlorination and Peroxidation Inhibitor Screening Assay Kit

Cayman’s MPO Inhibitor Screening Assay Kit provides convenient fluorometric methods for screening inhibitors to both the chlorination and peroxidation activities of MPO. The chlorination assay utilizes a non-fluorescent thiomorphiline-based probe (NBD-TM), which is selectively cleaved by hypochlorite (-OCl) to yield the highly fluorescent compound, NBD.¹ Fluorescence intensity is analyzed using an excitation wavelength of 500 nm and an emision wavelength of 550 nm. The peroxidation assay utilizes the peroxidase component of MPO, where a single two-electron oxidation of native enzyme (MPO) to compound I (MPO-I) is followed by two successive one-electron reductions back to native enzyme by compound II (MPO-II).² The reaction between hydrogen peroxide and 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) produces the highly fluorescent compound resorufin. Resorufin fluorescence is analyzed using an excitation wavelength of 530 nm and an emission wavelength of 590 nm.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

1. Pierzchala, K., Pieta, M., Rola, M., et al. Fluorescent probes for monitoring myeloperoxidase-derived hypochlorous acid: A comparative study. Scientific Reports 12, 9314 (2022).

2. Malle, E., Furtmüller, P.G., Sattler, W., et al. Myeloperoxidase: A target for new drug development? Br. J. Pharmacol. 152(6), 838-854 (2007).