A colorimetric method for rapid total protein quantification
Features
  • Measure total protein concentration
  • Assay range: 5.6-32 µg/ml
  • Plate-based colorimetric measurement (595 nm)
Related Products
Alternative(s)
701780Protein Determination (BCA) Kit
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Product Type

Assay & Reagent Kits
Assay Kits
Colorimetric Assays

Research Area

Protein Determination Kit

Item No. 704002

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Limit of Detection
5.6 µg/ml
Origin
Animal/Bovine
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    480 wells

    $85

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    Product Description

    The Cayman Chemical Protein Determination Kit is a microplate based, colorimetric method for rapid total protein quantification. Based on the well-known Bradford method,1 it takes advantage of the color change of Coomassie® Dye when it binds to proteins in acidic medium. When the dye binds, there is an immediate shift of the absorption maximum from 465 to 595 nm with a simultaneous change in color from brown to blue.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254 (1976).