A reducing co-substrate for heme peroxidases
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TMPD (hydrochloride)

Item No. 70455

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N,N,N',N'-tetramethyl-1,4-benzenediamine, dihydrochloride
CAS Number
637-01-4
Synonyms
  • Wurster’s Reagent
  • N,N,N',N'-Tetramethyl-p-Phenylenediamine
Molecular Formula
C10H16N2 • 2HCl
Formula Weight
237.2
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMSO: 1.7 mg/mlPBS (pH 7.2): 14.3 mg/ml
λmax
263, 308 nm
SMILES
CN(C)C1=CC=C(N(C)C)C=C1.Cl.Cl
InChi Code
InChI=1S/C10H16N2.2ClH/c1-11(2)9-5-7-10(8-6-9)12(3)4;;/h5-8H,1-4H3;2*1H
InChi Key
FBHKTSXMTASXFJ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
Room temperature
Shipping
Room temperature in continental US; may vary elsewhere
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    5 g

    $38

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    Product Description

    TMPD is an easily oxidizable compound that serves as a reducing co-substrate for heme peroxidases.1 TMPD undergoes one-electron oxidation by the heme peroxidase higher oxidation states (compounds I and II) producing a highly colored product that absorbs at 611 nm.2 Thus, the stoichiometry of oxidation is 2 moles of TMPD oxidized per mole of hydroperoxide reduced by the peroxidase. The extinction coefficient of the oxidized TMPD at 611 nm is 12,200.2 TMPD is also used for the detection of peroxidases on polyacrylamide gels.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Van der Ouderaa, F.J., Buytenhek, M., Nugteren, D.H., et al. Purification and characterisation of prostaglandin endoperoxide synthetase from sheep vesicular glands. Biochim. Biophys. Acta 487(2), 315-331 (1977).

    2. Kulmacz, R.J., and Lands, W.E.M. Requirements for hydroperoxide by the cyclooxygenase and peroxidase activities of prostaglandin H synthase. Prostaglandins 25(4), 531-540 (1983).

    3. Butler, M.J., and Lachance, M.A. The use of N,N,N',N'-tetramethylphenylenediamine to detect peroxidase activity on polyacrylamide electrophoresis gels. Anal. Biochem. 162(2), 443-445 (1987).