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Superoxide Dismutase Assay Kit

Item № 706002
product image
     96 wells $225.00 0.00
     480 wells $490.00 0.00

Pricing updated 2019-07-18. Prices are subject to change without notice.

  • Measure copper/zinc, manganese, and iron SOD in tissues, plasma, serum, and cell lysates
  • Assay 41 samples in duplicate
  • Measure SOD activity down to 0.005 U/ml
  • SOD Assay Kit

Significant amounts of superoxide dismutase (SOD) in cellular and extracellular environments are crucial for the prevention of diseases linked to oxidative stress. Mutations in SOD account for approximately 20% of familial amyotrophic lateral sclerosis (ALS) cases. SOD also appears to be important in the prevention of other neurodegenerative disorders such as Alzheimer’s, Parkinson’s, and Huntington’s Diseases.1,2 The reaction catalyzed by SOD is extremely fast, having a turnover of 2 x 106 M−1sec−1 and the presence of sufficient amounts of the enzyme in cells and tissues typically keeps the concentration of superoxide very low.3 Quantification of SOD activity is therefore essential in order to fully characterize the antioxidant capabilities of a biological system. The Cayman Chemical SOD Assay kit is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates, and cell lysates. SOD activity is assessed by measuring the dismutation of superoxide radicals generated by xanthine oxidase and hypoxanthine in a convenient 96 well format. The standard curve generated using this enzyme provides a means to accurately quantify the activity of all three types of SOD (Cu/Zn, Mn, and FeSOD). Each kit contains sufficient reagents to assay 41 samples in duplicate and includes assay buffer, sample buffer, radical detector, SOD (standard), xanthine oxidase, a 96 well plate, and complete instructions.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • SOD Assay Kit
  • Animal/Bovine

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Maier, C.M., and Chan, P.H. Role of superoxide dismutases in oxidative damage and neurodegenerative disorders Neuroscientist 8(4), 323-334 (2002).

2. Liu, D. The roles of free radicals in amyotrophic lateral sclerosis Journal of Molecular Neuroscience 7, 159-167 (1996).

3. Malström, B., Andreasson, L., and Reinhammer, B. XIIB The Enzymes 533 (1975).

Product Citations

Wen, J.J., and Garg, N.J. Manganese superoxide dismutase deficiency exacerbates the mitochondrial ROS production and oxidative damage in Chagas disease PLoS Negl. Trop. Dis. 12(7), e0006687 (2018).

Adeyemi, K.D., Shittu, R.M., Sabow, A.B., et al. Influence of diet and postmortem ageing on oxidative stability of lipids, myoglobin and myofibrillar proteins and quality attributes of Gluteus Medius muscle in goats PLOS One 11(5), e0154603 (2016).

Adeyemi, K.D., Shittu, R.M., Sabow, A.B., et al. Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats J. Anim. Sci. Technol. 58(23), (2016).

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Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Superoxide Dismutase Assay Kit

View all analysis workbooks

View the pre-configured analysis tool for Superoxide Dismutase Assay Kit on MyAssays.com

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Cayman Contract Services

We work with scientists to accelerate their research, drug discovery, and drug development needs through our expertise in the following areas:

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640