Superoxide Dismutase Assay Kit

Cayman’s Superoxide Dismutase Assay Kit provides a convenient method of measuring SOD activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates. The assay relies on xanthine oxidase to produce superoxide radicals, which then reduce a tetrazolium salt to a formazan dye, detectable at 450 nm. SOD in the sample inhibits this reduction by catalyzing the dismutation of superoxide radicals. Thus, the degree of inhibition is directly proportional to SOD activity. The SOD Assay Kit measures all three types of SOD (Cu/Zn-, Mn-, and Fe-SOD). Mitochondrial Mn-SOD can be assayed separately following the procedure outlined in the kit booklet.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

Biyashev, D., Siwicka, Z.E., Onay, U.V., et al. Topical application of synthetic melanin promotes tissue repair. NPJ Regen. Med. 8(1), 61 (2023).

Bilgic, H.A., Kilic, B., Kockaya, B.D., et al. Oxidative stress stimulation leads to cell-specific oxidant and antioxidant responses in airway resident and inflammatory cells. Life Sci. 315, 121358 (2023).

Selvakumar, G.P., Ahmed, M.E., Thangavel, R., et al. A role for glia maturation factor dependent activation of mast cells and microglia in MPTP induced dopamine loss and behavioural deficits in mice. Brain Behav. Immun. 87, 429-443 (2020).

Bernabeu-Wittel, M., Gómez-Díaz, R., González-Molina, Á., et al. Oxidative stress, telomeresShortening, and apoptosis associated to sarcopenia and frailty in patients with multimorbidity. J. Clin. Med. 9(8), 2669 (2020).

Ouyang, B., Li, Z., Ji, X., et al. The protective role of lutein on isoproterenol-induced cardiac failure rat model through improving cardiac morphology, antioxidant status via positively regulating Nrf2/HO-1 signalling pathway. Pharm. Biol. 57(1), 529-535 (2019).

Wiecek, M., Szymura, J., Maciejczyk, M., et al. Anaerobic Exercise-Induced Activation of Antioxidant Enzymes in the Blood of Women and Men. Front. Physiol. 9, 1006 (2018).

Chang, C.-W., Chen, C.-Y., Yen, C.-C., et al. Repressed exercise-induced hepcidin levels after danggui buxue tang supplementation in male recreational runners. Nutrients 10(9), E1318 (2018).

Wen, J.J., and Garg, N.J. Manganese superoxide dismutase deficiency exacerbates the mitochondrial ROS production and oxidative damage in Chagas disease. PLoS Negl. Trop. Dis. 12(7), e0006687 (2018).

Niraula, P., Ghimire, S., Lee, H., et al. Ilex paraguariensis extends lifespan and increases an ability to resist environmental stresses in Drosophila. Rejuvenation Res. 21(6), 497-505 (2018).

Lee, H.Y., Kim, S.W., Lee, G.H., et al. Curcumin and Curcuma longa L. extract ameliorate lipid accumulation through the regulation of the endoplasmic reticulum redox and ER stress. Sci. Rep. 7(1), 6513 (2017).

Wang, M.-Y., Srinivasan, M., Dasari, D., et al. Antioxidant activity of Yichun Blue Honeysuckle (YBHS) berry counteracts CCl4-Induced toxicity in liver injury model of mice. Antioxidants 6(3), 50 (2017).

Lee, G.-H., Lee, H.-Y., Choi, M.-K., et al. Protective effect of Curcuma longa L. extract on CCl4-induced acute hepatic stress. BMC Res. Notes 10(1), 77 (2017).

Alarifi, S., Ali, H., Alkahtani, S., et al. Regulation of apoptosis through bcl-2/bax proteins expression and DNA damage by nano-sized gadolinium oxide. Int. J. Nanomedicine 12, 4541-4551 (2017).

Kowalska, K., and Milnerowicz, H. The influence of age and gender on the pro/antioxidant status in young healthy people. Ann. Clin. Lab. Sci. 46(5), 480-488 (2016).

Velviranli, M., Okudan, N., Revan, S., et al. Repeated supramaximal exercise-induced oxidative stress: Effect of β-alanine plus creatine supplementation. Asian J. Sports Med. 7(1), e26843 (2016).

Yoon, S.P., and Kim, J. Poly(ADP-ribose) polymerase 1 contributes to oxidative stress through downregulation of sirtuin 3 during cisplatin nephrotoxicity. Anat. Cell Biol. 49, 165-176 (2016).

Salvi, A., Patki, G., Khan, E., et al. Relationship between advanced glycation end products and increased lipid peroxidation in semen of diabetic men. Oxid. Med. Cell. Longev. 5059043 (2016).

Kleniewska, P., Hoffmann, A., Pniewska, E., et al. The influence of probiotic Lactobacillus casei in combination with prebiotic inulin on the antioxidant capacity of human plasma. Oxid. Med. Cell. Longev. 1340903, (2016).

Wang, X.X., Edelstein, M.H., Gafter, U., et al. G protein-coupled bile acid receptor TGR5 activation inhibits kidney disease in obesity and diabetes. J. Am. Soc. Nephrol. 27(5), 1362-1378 (2016).

Adeyemi, K.D., Shittu, R.M., Sabow, A.B., et al. Influence of diet and postmortem ageing on oxidative stability of lipids, myoglobin and myofibrillar proteins and quality attributes of Gluteus Medius muscle in goats. PLOS One 11(5), e0154603 (2016).

Vitti, A., Pellegrini, E., Nali, C., et al. Trichoderma harzianum T-22 induces systemic resistance in tomato infected by Cucumber mosaic virus. Front. Plant Sci. 7(1520), (2016).

Adeyemi, K.D., Shittu, R.M., Sabow, A.B., et al. Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats. J. Anim. Sci. Technol. 58(23), (2016).

Metta, S., Basalingappa, D.R., Uppala, S., et al. Erythrocyte antioxidant defenses against cigarette smoking in ischemic heart disease. J. Clin. Diagn. Res. 9(6), BC08-BC11 (2015).

Dove, R.E., Leong-Smith, P., Roos-Engstrand, E., et al. Cigarette smoke-induced induction of antioxidant enzyme activities in airway leukocytes is absent in active smokers with COPD. Eur. Clin. Respir. J. 2, (2015).

Albaayit, S.F., Abba, Y., Rasdee, A., et al. Effect of Clausena excavata Burm. f. (Rutaceae) leaf extract on wound healing and antioxidant activity in rats. Drug Des. Devel. Ther. 13:9, 3507-3518 (2015).

Rouhollahi, E., Moghadamtousi, S.Z., Hakiaghaalipour, F., et al. Curcuma purpurascens BI. rhizome accelerates rat excisional wound healing: involvement of Hsp70/Bax proteins, antioxidant defense, and angiogenesis activity. Drug Des. Devel. Ther. 9, 5805-5813 (2015).

Karthivashan, G., Arulselvan, P., Alimon, A.R., et al. Competing role of bioactive constituents in Moringa oleifera extract and conventional nutrition feed on the performance of Cobb 500 broilers. Biomed Res. Int. 970398, (2015).

Albaayit, S.F., Abba, Y., Rasedee, A., et al. Effect of Clausena excavata Burm. f. (Rutaceae) leaf extract on wound healing and antioxidant activity in rats. Drug Des. Devel. Ther. 9, 3507-3518 (2015).

Almerich-Silla, J.M., Montiel-Company, J.M., Pastor, S., et al. Oxidative stress parameters in saliva and its association with periodontal disease and types of bacteria. Dis. Markers 653537 (2015).

Christensen, L.L., Selman, C., Blount, J.D., et al. Plasma markers of oxidative stress are uncorrelated in a wild mammal. Ecol. Evol. 5(21), 5096-5108 (2015).

Niedzwiedz, A., and Jaworski, Z. Oxidant-antioxidant status in the blood of horses with symptomatic recurrent airway obstruction (RAO). J. Vet. Intern. Med. 28(6), 1845-1852 (2014).

Kadir, F.A., Kassim, N.M., Abdulla, M.A., et al. PASS-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats. Sci. World J. 301879 (2014).

Dhall, S., Do, D.C., Garcia, M., et al. Generating and reversing chronic wounds in diabetic mice by manipulating wound redox parameters. J. Diabetes Res. 562625, (2014).

González-Peña, D., Angulo, J.F., Vallejo, S., et al. High-cholesterol diet enriched with onion affects endothelium-dependent relaxation and NADPH oxidase activity in mesenteric microvessels from Wistar rats. Nutr. Metab. (Lond) 11:57, (2014).

Faisal, M., Saquib, Q., Alatar, A.A., et al. Phytotoxic hazards of NiO-nanoparticles in tomato: A study on mechanism of cell death. J. Hazard. Mater. 250-251, 318-332 (2013).

Guo, C.H., Chen, P.C., Hsu, G.S., et al. Zinc supplementation alters plasma aluminum and selenium status of patients undergoing dialysis: A pilot study. Nutrients 5(4), 1456-1470 (2013).

Eberle, C., Merki, E., Yamashita, T., et al. Maternal immunization affects in utero programming of insulin resistance and type 2 diabetes. PLoS One 7(9), e45361 (2012).

Yuan, C.Y., Lee, Y.J., and Hsu, G.S. Aluminum overload increases oxidative stress in four functional brain areas of neonatal rats. J. Biomed. Sci. 19, 51 (2012).

Liu, I.M., Tzeng, T.F., Liou, S.S., et al. Beneficial effect of traditional chinese medicinal formula danggui-shaoyao-san on advanced glycation end-product-mediated renal injury in streptozotocin-diabetic rats. Evid. Based Complement. Alternat. Med. 2012, 140103 (2012).

Sullivan-Gunn, M.J., Campbell-O'Sullivan, S.P., Tisdale, M.J., et al. Decreased NADPH oxidase expression and antioxidant activity in cachectic skeletal muscle. J. Cachexia Sarcopenia Muscle 2(3), 181-188 (2011).