For measurement of the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysate samples
Features
  • Measure the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysates
  • Assay 41 samples in duplicate
  • Measure antioxidant capacity in Trolox equivalents down to 44 μM
  • Plate-based colorimetric measurement (750 or 405 nm)
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Antioxidant Assay Kit

Item No. 709001

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Assay Range
0.068-0.495 nM
Origin
Animal/Horse
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $329

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    Product Description

    Cayman’s Antioxidant Assay can be used to measure the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysates. Aqueous- and lipid-soluble antioxidants are not separated in this protocol, thus the combined antioxidant activities of all its constituents including vitamins, proteins, lipids, glutathione, uric acid, etc. are assessed. The assay relies on the ability of antioxidants in the sample to inhibit the oxidation of ABTS® (2,2’-Azino-di-[3-ethylbenzthiazoline sulphonate]) to ABTS®•+ by metmyoglobin. The amount of ABTS®•+ produced can be monitored by reading the absorbance at 750 nm or 405 nm. Under the reaction conditions used, the antioxidants in the sample cause suppression of the absorbance at 750 nm or 405 nm to a degree which is proportional to their concentration.1,2,3,4 The capacity of the antioxidants in the sample to prevent ABTS® oxidation is compared with that of Trolox, a water-soluble tocopherol analogue, and is quantified as millimolar Trolox equivalents.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Miller, N.J., Rice-Evans, C., Davies, M.J., et al. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin. Sci. (Lond.) 84, 407-412 (1993).

    2. Miller, N.J., and Rice-Evans, C. Factors influencing the antioxidant activity determined by the ABTS + radical cation assay. Free Radic. Res. 26, 195-199 (1997).

    3. Miller, N.J., Rice-Evans, C., and Davies, M.J. A new method for measuring antioxidant activity. Biochem. Soc. Trans. 21, 95S-95S (1993).

    4. Rice-Evans, C., and Miller, N. Total antioxidant status in plasma and body fluids. Methods Enzymol. 234(24), 279-293 (1994).

    Product Citations

    Gozdowska, M., Sokolowska, E., Pomianowski, K., et al. Melatonin and cortisol as components of the cutaneous stress response system in fish: Response to oxidative stress. Comp. Biochem. Physiol. Part A 268, 111207 (2022).

    Bomble, P., and Nath, B.B. Differential manifestation of RONS and antioxidant enzymes in response to singular versus combinatorial stress in Chironomus ramosus. Stress Biol. 2(1), 56 (2022).

    Bernabeu-Wittel, M., Gómez-Díaz, R., González-Molina, Á., et al. Oxidative stress, telomeresShortening, and apoptosis associated to sarcopenia and frailty in patients with multimorbidity. J. Clin. Med. 9(8), 2669 (2020).

    Wen, J.J., Yin, Y.W., and Garg, N.J. PARP1 depletion improves mitochondrial and heart function in Chagas disease: Effects on POLG dependent mtDNA maintenance. PLoS Pathog. 14(5), e1007065 (2018).

    Peng, K.-T., Tsai, M.-H., Lee, C.-W., et al. Dysregulated expression of antioxidant enzymes in polyethylene particle-induced periprosthetic inflammation and osteolysis. PLOS One 13(8), e0202501 (2018).

    Vieira, G.d.L.T., Lossie, A.C., Lay, D.C., Jr., et al. Preventing, treating, and predicting barbering: A fundamental role for biomarkers of oxidative stress in a mouse model of Trichotillomania. PLoS One 12(4), e0175222 (2017).

    Kephart, W.C., Mumford, P.W., Mao, X., et al. The 1-week and 8-month effects of a ketogenic diet or ketone salt supplementation on multi-organ markers of oxidative stress and mitochondrial function in rats. Nutrients 9(9), e1019 (2017).

    Vieira, G.L.T., Lossie, A.C., Lay, D.C., Jr., et al. Preventing, treating, and predicting barbering: A fundamental role for biomarkers of oxidative stress in a mouse model of Trichotillomania. PLoS One 12(4), e0175222 (2017).

    Tsamesidis, I., Fozza, C., Vagdatli, E., et al. Total antioxidant capacity in Mediterranean β-thalassemic patients. Adv. Clin. Exp. Med. 26(5), 789-793 (2017).

    Salvi, A., Patki, G., Khan, E., et al. Relationship between advanced glycation end products and increased lipid peroxidation in semen of diabetic men. Oxid. Med. Cell. Longev. 5059043 (2016).

    Velviranli, M., Okudan, N., Revan, S., et al. Repeated supramaximal exercise-induced oxidative stress: Effect of β-alanine plus creatine supplementation. Asian J. Sports Med. 7(1), e26843 (2016).

    Meléndez García , R., Arredondo Zamarripa, D., Arnold, E., et al. Prolactin protects retinal pigment epithelium by inhibiting sirtuin 2-dependent cell death. EBioMedicine 7, 35-49 (2016).

    McKnight, L.L., Eyre, R., Gooding, M.A., et al. Dietary mannoheptulose increases fasting serum glucagon like peptide-1 and post-prandial serum ghrelin concentrations in adult beagle dogs. Animals (Basel) 5(2), 442-454 (2015).

    Mesa, M.D., Olza, J., Gonzalez-Anton, C., et al. Changes in oxidative stress and inflammatory biomarkers in fragile adults over fifty years of age and in elderly people exclusively fed enteral nutrition. Oxid. Med. Cell. Longev. 5709312, (2015).

    Christensen, L.L., Selman, C., Blount, J.D., et al. Plasma markers of oxidative stress are uncorrelated in a wild mammal. Ecol. Evol. 5(21), 5096-5108 (2015).

    Almerich-Silla, J.M., Montiel-Company, J.M., Pastor, S., et al. Oxidative stress parameters in saliva and its association with periodontal disease and types of bacteria. Dis. Markers 653537 (2015).

    Limeraki, E., Eleftheriou, P., Gasparis, G., et al. Cortisol levels and serum antioxidant status following chemotherapy. Sci. Res. 3(8), 512-517 (2011).

    Jackson, R., Ramos, C.L., Gupta, C., et al. Exercise decreases plasma antioxidant capacity and increases urinary isoprostanes of IPF patients. Respir. Med. 104(12), 1919-1928 (2010).

    Mahfouz, R., Sharma, R., Sharma, D., et al. Diagnostic value of the total antioxidant capacity (TAC) in human seminal plasma. Fertil. Steril. 91(3), 805-811 (2009).

    Agarwal, A., Desai, N.R., Makker, K., et al. Effects of radiofrequency electromagnetic waves (RF-EMW) from cellular phones on human ejaculated semen: An in vitro pilot study. Fertil Steril. 92(4), 1318-1325 (2009).