A colorimetric assay for the detection of COX activity in tissue homogenates and purified enzyme preparations
Features
  • Measure COX activity in tissue homogenates and purified enzyme preparations
  • Measure the peroxidase component of the COX
  • Assay 23 samples in duplicate or 11 samples when using optional COX-1 & COX-2 inhibitors
  • Plate-based colorimetric measurement (590 nm)
  • Includes isozyme-specific inhibitors for distinguishing COX-2 activity from COX-1 activity
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COX Activity Assay Kit

Item No. 760151

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • ​Cyclooxygenase
  • PGHS
  • Prostaglandin H Synthase
Origin
Animal/Sheep
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $480

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    Product Description

    Cayman’s COX Activity Assay Kit measures the peroxidase activity of COX. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N’,N’-tetramethyl-p-phenylenediamine (TMPD) at 590 nm.1 It can be used with tissue homogenates and purified enzyme preparations. The kit includes isozyme-specific inhibitors for distinguishing COX-2 activity from COX-1 activity.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kulmacz, R.J., and Lands, W.E.M. Requirements for hydroperoxide by the cyclooxygenase and peroxidase activities of prostaglandin H synthase. Prostaglandins 25(4), 531-540 (1983).

    Product Citations

    Wahle, B.M., Hawley, E.T., He, Y., et al. Chemopreventative celecoxib fails to prevent schwannoma formation or sensorineural hearing loss in genetically engineered murine model of neurofibromatosis type 2. Oncotarget 9(1), 718-725 (2018).

    Tamayose, C.I., Romoff, P., Toyama, D.O., et al. Non-clinical studies for evaluation of 8-C-rhamnosyl apigenin purified from Peperomia obtusifolia against acute edema. Int. J. Mol. Sci. 18(9), E1972 (2017).

    Kugathas, S., Audouze, K., Ermler, S., et al. Effects of common pesticides on prostaglandin D2 (PGD2) Inhibition in SC5 mouse sertoli cells, evidence of binding at the COX-2 active site, and implications for endocrine disruption. Environ. Health Perspect. 124(4), 452-459 (2016).

    Ercan, S., Kencebay, C., Basaranlar, G., et al. Induction of omega 6 inflammatory pathway by sodium metabisulfite in rat liver and its attenuation by ghrelin. Lipids Health Dis. 14:7, (2015).

    Dai, M., and Shi, X. Fibro-vascular coupling in the control of cochlear blood flow. PLoS One 6(6), e20652 (2011).

    Toller, I.M., Hitzler, I., Sayi, A., et al. Prostaglandin E2 prevents Helicobacter-induced gastric preneoplasia and facilitates persistent infection in a mouse model. Gastroenterology 138(4), 1467 (2010).