Lipoxygenase Inhibitor Screening Assay Kit

Cayman’s Lipoxygenase Inhibitor Screening Assay Kit detects and measures the hydroperoxides produced in the lipoxygenation reaction using a purified LO. The detection reaction is equally sensitive to hydroperoxides at various positions within the fatty acid, and will work with fatty acids of any carbon length. It is thus a general detection method for LO, and can be used to screen libraries of compounds for those which inhibit LO enzymes.

Provided in this kit is arachidonate 15-LO purified from soybean, which can be used to screen test compounds. Alternatively, if other purified LOs (provided by the user) are to be used for screening, the included enzyme can be used as a control. Both arachidonic and linoleic acids are included as substrates. The preferred substrate for screening will depend on the LO being tested. It is recommended to use the fatty acid substrate best suited for the LO being tested.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

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