For the measurement of cytosolic and extracellular PAF-AH activity
Features
  • Measure PAF-AH activity in plasma, serum, tissue homogenates, and cell lysates
  • Assay 23 samples in duplicate
  • Measure PAF-AH activity down to 0.02 U/ml
  • Plate-based colorimetric measurement (405-415 nm)
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PAF Acetylhydrolase Assay Kit

Item No. 760901

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Lp-PLA2
  • PAF-AH
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $269

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    Product Description

    Platelet-activating factor (PAF) is a biologically active phospholipid synthesized by a variety of cells upon stimulation. PAF is converted to the biologically inactive lyso-PAF by the enzyme PAF acetylhydrolase (PAF-AH). Plasma PAF-AH is highly selective for phospholipids with very short acyl groups at the sn-2 position and is associated with lipoproteins.1 The Cayman Chemical PAF acetylhydrolase assay kit provides an accurate and convenient method for measurement of PAF-AH activity. The assay uses 2-thio PAF which serves as a substrate for PAF-AH.2 Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB; Ellman’s reagent). The dynamic range of the assay is only limited by the accuracy of the absorbance measurement. Most plate readers are linear to an absorbance of 1.2. The detection range of the assay is from 0.02 to 0.2 µmol/min/ml of PAF-AH activity which is equivalent to an absorbance increase of 0.01 to 0.1 per minute. Each kit contains assay buffer, DTNB, 2-thio PAF (substrate), human PAF-AH standard, a 96 well plate, and complete instructions.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Stafforini, D.M., Prescott, S.M., and McIntyre, T.M. Human plasma platelet-activating factor acetylhydrolase. The Journal of Biological Chemisty 262(9), 4223-4230 (1987).

    2. Aarsman, A.J., Neys, F.W., and Van den Bosch, H. Catabolism of platelet-activating factor and its acyl analog. Differentiation of the activities of lysophospholipase and platelet-activating-factor acetylhydrolase. Eur. J. Biochem. 200(1), 187-193 (1991).

    Product Citations

    Milasan, A., Jean, G., Dallaire, F., et al. Apolipoprotein A-I modulates atherosclerosis through lymphatic vessel-dependent mechanisms in mice. J. Am. Heart Assoc. 6(9), e006892 (2017).

    Woudberg, N.J., Goedecke, J.H., Blackhurst, D., et al. Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution. Lipids Health Dis. 15(92), (2016).

    Gong, H.-p., Du, Y.-m., Zhong, L.-n., et al. Plasma lipoprotein-associated phospholipase A2 in patients with metabolic syndrome and carotid atherosclerosis. Lipids Health Dis. 10:13, (2011).

    Bellido Reyes, Y.A., Shimoyama, T., Akamatsu, H., et al. MCI-186 (edaravone), a free radical scavenger, attenuates ischemia-reperfusion injury and activation of phospholipase A2 in an isolated rat lung model after 18 h of cold preservation. Eur. J. Cardiothorac. Surg. 29(3), 304-311 (2006).