A NOX inhibitor
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Diphenyleneiodonium (chloride)

Item No. 81050

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
dibenziodolium chloride
CAS Number
4673-26-1
Synonyms
  • DPI
Molecular Formula
C12H8I • Cl
Formula Weight
314.5
Purity
≥98%
Formulation
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMSO: 500 µg/mlEthanol: <100 µg/mlMethanol: <100 µg/ml
SMILES
C12=C(C3=C([I+]2)C=CC=C3)C=CC=C1.[Cl-]
InChi Code
InChI=1S/C12H8I.ClH/c1-3-7-11-9(5-1)10-6-2-4-8-12(10)13-11;/h1-8H;1H/q+1;/p-1
InChi Key
FCFZKAVCDNTYID-UHFFFAOYSA-M
Shipping & Storage Information
Storage
Room temperature
Shipping
Room temperature in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    5 mg

    $50

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    10 mg

    $60

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    Cayman Chemical
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    Product Description

    Diphenyleneiodonium (DPI) is an inhibitor of NADPH oxidase (NOX; EC50 = 0.1 µM in HeLa cells).1 It also inhibits nitric oxide synthase (NOS; IC50 = 0.05 µM in isolated mouse peritoneal macrophages).2 DPI (10, 50, and 100 µM) induces the production of reactive oxygen species (ROS) in, and apoptosis of, human umbilical vein endothelial cells (HUVECs).3 It inhibits NETosis induced by phorbol 12-myristate 13-acetate (PMA; Item No. 10008014) in isolated human neutrophils when used at a concentration of 10 µM.4 DPI (2 mg/kg) reduces tumor growth in HT-29 and LS 174T colon cancer mouse xenograft models.5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Morré, D.J. Preferential inhibition of the plasma membrane NADH oxidase (NOX) activity by diphenyleneiodonium chloride with NADPH as donor. Antioxid. Redox Signal. 4(1), 207-212 (2002).

    2. Stuehr, D.J., Fasehun, O.A., Kwon, N.S., et al. Inhibition of macrophage and endothelial cell nitric oxide synthase by diphenyleneiodonium and its analogs. FASEB J. 5, 98-103 (1991).

    3. Balcerczyk, A., Soszynski, M., Rybaczek, D., et al. Induction of apoptosis and modulation of production of reactive oxygen species in human endothelial cells by diphenyleneiodonium. Biochem. Pharmacol. 69(8), 1263-1273 (2005).

    4. Ostafin, M., Pruchniak, M.P., Ciepiela, O., et al. Different procedures of diphenyleneiodonium chloride addition affect neutrophil extracellular trap formation. Anal. Biochem. 509, 60-66 (2016).

    5. Doroshow, J.H., Gaur, S., Markel, S., et al. Effects of iodonium-class flavin dehydrogenase inhibitors on growth, reactive oxygen production, cell cycle progression, NADPH oxidase 1 levels, and gene expression in human colon cancer cells and xenografts. Free Radic. Biol. Med. 57, 162-175 (2013).

    Product Citations

    Ting, K.K.Y., Ibrahim, H.M., Gulati, N., et al. Intracellular accumulation of free cholesterol in macrophages triggers a PARP1 response to DNA damage and PARP1 impairs lipopolysaccharide-induced inflammatory response. PLoS One 20(3), e0318267 (2025).

    Nakabo, S., Ohmura, K., Akizuki, S., et al. Activated neutrophil carbamylates albumin via the release of myeloperoxidase and reactive oxygen species regardless of NETosis. Mod. Rheumatol. 30(2), 345-349 (2019).

    Xu, S., Catapang, A., Braas, D., et al. A precision therapeutic strategy for hexokinase 1-null, hexokinase 2-positive cancers. Cancer & Metab. 6:7, (2018).